Research Articles
Permanent URI for this collection
Browse
Recent Submissions
Item Next generation sequencing and genetic analyses reveal factors driving evolution of sweetpotato viruses in Uganda(MDPI, 2024-09-26) Adero, Joanne; Wokorach, Godfrey; Stomeo, Francesca; Yao, Nasser; Machuk, Eunice; Njuguna, Joyce; Byarugaba, Denis K.; Kreuze, Jan; Yencho, G. Craig; Otema, Milton A.; Yad, Benard; Kitavi, MercySweetpotato (Ipomoea batatas L.) is an essential food crop globally, especially for farmers facing resource limitations. Like other crops, sweetpotato cultivation faces significant production challenges due to viral infections. This study aimed to identify and characterize viruses affecting sweetpotato crops in Uganda, mostly those associated with sweetpotato virus disease (SPVD). Infected leaf samples were collected from farmers’ fields in multiple districts spanning three regions in Uganda. MiSeq, a next-generation sequencing platform, was used to generate reads from the viral nucleic acid. The results revealed nine viruses infecting sweetpotato crops in Uganda, with most plants infected by multiple viral species. Sweet potato pakakuy and sweet potato symptomless virus_1 are reported in Uganda for the first time. Phylogenetic analyses demonstrated that some viruses have evolved to form new phylogroups, likely due to high mutations and recombination, particularly in the coat protein, P1 protein, cylindrical inclusion, and helper component proteinase regions of the potyvirus. The sweet potato virus C carried more codons under positive diversifying selection than the closely related sweet potato feathery mottle virus, particularly in the P1 gene. This study provides valuable insights into the viral species infecting sweetpotato crops, infection severity, and the evolution of sweet potato viruses in Uganda.Item Structural equation model for a relationship between family support systems and alcohol addiction recovery: mediation effect of continuing care services.(Journal of Advanced Psychology, 2024-08-09) Kyazze, Richard; Natuhamya, Charles; Mwebesa, Edson; Kiyingi, Frank; Musoke, Miph; Tumwesigye, Nazarius MbonaPurpose: Alcohol use disorder continues to be a public health concern, and even after treatment, the relapse rate is still high. The family support systems are closely related to alcohol addiction recovery. However, studies on psychological mechanisms between the two are rare. Therefore, we aimed to explore the mediating role of continuing care services in the pathway between family support systems and alcohol addiction recovery among individuals with alcohol use disorder. Methods: A total of 196 participants from 5 treatment centers (Butabika National Referral Mental Hospital, Serenity Centre, Recovery Solutions, National Care Centre, and Hope and Beyond) were selected from a target population of 400 individuals. The structural equation modeling approach was used to assess the study objective. Results: The average alcohol addiction recovery status score was 3.03 (SD = 0.95). The mediation analysis indicated that continuing care services mediated the pathway between family support systems and alcohol addiction recovery. Unique Contribution to Theory, Practice and Policy: Motivational enhancement therapy, mutual health support, and cognitive behavioral therapy are key factors in the relationship between family support systems and alcohol addiction recovery.Item A Comparative study of photon radiation-shielding properties of different glass types for use in health facilities(University of Warwick and Monash University, 2024-09-11) Nyangoma, Judith; Oryema, BoscoThe usage of X-ray generating devices and gamma-ray sources such as 60Co and 137Cs for medical diagnostic and therapeutic applications has increased globally. However, exposure to radiation from these sources can cause detrimental effects on biological tissues. Thus, to optimise radiation safety, effective radiation shields are required. This study used the photon shielding and dosimetry (PSD) software to simulate and compare the photon shielding properties of phosphate, bismuthate, tellurite, silicate and borate glass for use in medical facilities. The parameters investigated included mass attenuation coefficient (MAC), linear attenuation coefficient (LAC), half-value layer (HVL), mean-free path (MFP), and effective atomic number (Zeff). The results showed that bismuthate glass had the highest MAC and LAC values followed by tellurite, silicate, phosphate and borate glass respectively. It was also found that bismuthate glass had the lowest HVL and MFP values followed by tellurite, silicate, phosphate and borate glass. Since materials with high MAC and LAC and low HVL and MFP are associated with higher photon stoppage powers, bismuthate glass are better photon shielding materials compared to the rest of the glass examined in this study. Conversely, borate glass presented the least shielding potential compared to phosphate, silicate and tellurite glass.Item Knowledge, attitudes and practices towards tuberculosis: results of a community-based survey in the Karamoja subregion, North Eastern Uganda(Springer Nature, 2024-09-12) Kasozi, William; Mwebesa, Edson; Zawedde-Muyanja, Stella; Nsubuga, Tadeo; Musaazi, Joseph; Etwom, Alfred; Lemukol, James; Sagaki, Patrick; Tino, Salome; Achar, Cerino; Stavia, Turyahabwe; Murungi, Miriam; Rutta, Edmund; Nabukenya-Mudiope, Mary G.Background: The Karamoja subregion in north-eastern Uganda has very high Tuberculosis (TB)case notification rates and, until recently, had suboptimal treatment completion rates among patients diagnosed with TB. We evaluated community knowledge, attitudes and practices towards Tuberculosis in order to identify barriers to TB control in this region. Methods: From September to October 2022, we conducted a community-based survey in four districts in the Karamoja subregion. We collected data on TB knowledge, attitudes, and practices using a structured electronic questionnaire. We generated knowledge, attitude and practice scores. We used a survey-weighted zero-truncated modified Poisson model to assess the association between knowledge/attitude scores and respondents’ characteristics and a complementary log-log model to assess the association between practice scores and respondents’ characteristics. Data analysis was carried out using STATA version 14. Results: A total of 1927 respondents were interviewed. Of these, 55.5% were female, and 1320 (68.6%) had no formal education. Overall, 68.5% 95% CI (59.6–76.7%) had knowledge scores of ≥ 60%. Higher TB knowledge scores were associated with being employed (adjusted prevalence ratio, aPR = 1.22, 95% CI 1.04–1.42, p = 0.01) while being a wife in a household was associated with lower TB knowledge (aPR = 0.90, 95% CI 0.82–0.99, p = 0.03). Being 25–34 years old was associated with a positive attitude towards TB (PR = 1.06, 1.01–1.11, p = 0.01). Over 85% of respondents would go to the health facility immediately if they had TB signs and symptoms. Almost all respondents (98.6%) would start TB treatment immediately if diagnosed with the disease. Conclusion: More than two thirds of patients had good knowledge and practices towards TB which can be leveraged to improve uptake of TB control interventions in the region. Additional interventions to improve TB knowledge and practice should focus on specific segments within the communities e.g., older women in the households.Item An ethnobotanical survey of plant species used for medicinal purposes in Amuru district, northern Uganda(Ilia State University, 2024-09-12) Gum, Bornfree; Opoke, Robert; Akwongo, Betty; Oloya, Benson; Omony, John Bosco; Opiro, Robert; Andama, Morgan; Anywar, Godwin; Malinga, Geoffrey MaxwellBackground: Medicinal plants provide health care to rural communities that have limited access to modern medicine in Uganda. Thus, documenting medicinal plants is important for their sustainable utilisation and conservation of medicinal plants and associated indigenous knowledge. Methods: A cross-sectional ethnobotanical survey was conducted in August 2021 to assess the knowledge and use of medicinal plants in Lamogi Sub-County, Amuru district. We randomly sampled and interviewed 334 household heads using semi-structured questionnaires. Data was analysed using descriptive statistics, familiarity index (FI), fidelity level (FL) and informant consensus factor (ICF). Results: Most of the respondents (90%) had knowledge of medicinal plants used for treating ailments in their community (high ICF values above 0.9). Plant with the highest FI value was Gymnanthemum amygdalinum (Delile) Sch.Bip. indicating its wide use in the community. Chi-square tests showed significant associations between the knowledge of medicinal plants and education level, occupation, age, marital status and monthly income of respondents (p < 0.05). Forty-nine medicinal plants from 30 families were documented, with Fabaceae and Asteraceae having the most species. Leaves were the most frequently used plant parts (65.4%), followed by roots (13.6%). Herbal remedies were mostly prepared by crushing and smearing on the body, infusion, decoction and administered orally. Conclusion: The people of Amuru district have rich indigenous knowledge of diverse medicinal plants used for treating ailments. Most medicinal plants are harvested from the wild, threatening their survival and this requires putting in place practical conservation measures.Item Draft genome sequence of Acinetobacter haemolyticus strain MUWRP1017 isolated from the pus of a female inpatient at Bwera General Hospital in Uganda(American Society for Microbiology, 2024-08-20) Wokorach, Godfrey; Erima, Bernard; Alafi, Stephen; Kabatesi, Hope O.; Muhindo, Julius T.; Najjuka, Florence; Kiyengo, James; Kibuuka, Hannah; Musinguzi, Ambrose K.; Wabwire-Mangen, Fred; Byarugaba, Denis K.The bacterium Acinetobacter haemolyticus, with a genome size of 3.4 Mb, was isolated from a pus swab of a wound on the left lower limb above the ankle joint of a female patient. This strain carries the antimicrobial resistance genes cephalosporinase blaADC-25, oxallinase blaOXA-264, floR, and sul2 and other resistance and virulence genes.Item Impact of mass media campaigns on knowledge of malaria prevention measures among pregnant mothers in Uganda: a propensity score‑matched analysis(Springer Nature, 2024-08-24) Mwebesa, Edson; Awor, Susan; Natuhamya, Charles; Dricile, Ratib; Legason, Ismail D.; Okimait, David; Mangwi, Richard Ayiasi; Tumwesigye, Nazarius M.Background: Uganda grapples with a considerable malaria burden, reporting prevalence rates of over 33% in some regions. To address this, the Uganda Ministry of Health employs audiovisual platforms for disseminating malaria prevention messages. However, the impact of these messages on pregnant women’s knowledge of malaria prevention remains insufficiently explored. This paper therefore emphasizes the influence of audiovisual messages on the knowledge of malaria prevention measures among pregnant women in Uganda. Methods: Secondary data obtained from the Uganda Malaria Indicator Survey (MIS) 2018–2019 was used for this analysis. Women aged 15–49 were included in the study. A total of 8868 women were selected using a two-stage sample design. The two stages of selection included clusters and households. Women who were currently pregnant were included in the study, resulting in a weighted sample of 721 women. Propensity score-matched analysis was used to evaluate the impact of access to malaria messages on knowledge of prevention measures. Results: The study revealed that 39% [95% CI 34.0–44.2] of pregnant women were exposed to malaria messages before the survey. Those exposed had a 17.2% higher knowledge [ATT = 0.172; 95% CI 0.035–0.310] of using mosquito nets for prevention compared to those unexposed. Among women exposed, radios accounted for most form of access to mass media campaigns [64.8, 95% CI 57.0–71.8] followed by interpersonal communication [45.0, 95% CI 37.6–52.6], community health workers [38.8, 95% CI 29.6–48.8], community events [21.4, 95% CI 15.8–28.3], and social mobilization [18.3, 95% CI 12.7–25.8]. Conclusion: Results highlight the importance of radios in spreading important malaria prevention messages to pregnant women. Being exposed to these messages is linked to increased awareness and knowledge about the proper use of insecticide-treated bed nets (ITNs) for preventing malaria. This finding underscores the importance of evaluating different channels for mass media campaigns to ensure the effective delivery of information about malaria prevention to the intended audiences.Item Noteworthy bird records from south-west South Sudan game reserves(British Ornithologists' Club, 2023-09-07) Brooks, Daniel M.; Sutton, Jack; Kurchez, Laura A.; Garside, Adrian; Ejotre, Imran; Rice, Matt; Moeller, Michelle L.; Harris, Robert J.; Klee, Ivan De; Reeder, DeeAnn M.Avian camera trap image data from two game reserves in southwestern South Sudan produced three new country records, four other range extensions, and one filled a distributional gap between north-west Ethiopia and eastern Central African Republic. The study took place at Bangangai (31 traps, January 2015–February 2016) and Bire Kpatuos Game Reserves (52 traps, September 2015–August 2017). A total of 40 species (18 families) was recorded, including two Near Threatened species of global conservation concern: Crowned Eagle Stephanoaetus coronatus and White-naped Pigeon Columba albinucha. The occurrence of Black Goshawk Accipiter melanoleucus, Grey-throated Rail Canirallus oculeus and Nkulengu Rail Himantornis haematopus represent first records for South Sudan.Item Epauletted fruit bats display exceptionally high infections with a Hepatocystis species complex in South Sudan(Springer Nature, 2017-01-31) Schaer, Juliane; Perkins, Susan L.; Ejotre, Imran; Vodzak, Megan E.; Matuschewski, Kai; DeeAnn, M.Hepatocystis parasites are closely related to mammalian Plasmodium species, the causative agents of malaria. Despite the close phylogenetic relationship, Hepatocystis parasites lack the intermittent erythrocytic replication cycles, the signature and exclusive cause of malaria-related morbidity and mortality. Hepatocystis population expansion in the mammalian host is thought to be restricted to the pre-erythrocytic liver phase. Complete differentiation of first generation blood stages into sexual stages for subsequent vector transmission indicates alternative parasite/host co-evolution. In this study, we identified a region of exceptionally high prevalence of Hepatocystis infections in Old World fruit bats in South Sudan. Investigations over the course of five consecutive surveys revealed an average of 93 percent prevalence in four genera of African epauletted fruit bats. We observed a clear seasonal pattern and tolerance of high parasite loads in these bats. Phylogenetic analyses revealed several cryptic Hepatocystis parasite species and, in contrast to mammalian Plasmodium parasites, neither host specificity nor strong geographical patterns were evident. Together, our study provides evidence for Pan-African distribution and local high endemicity of a Hepatocystis species complex in Pteropodidae.Item Hepatocystis(Trends in Parasitology, 2020-08-21) Ejotre, Imran; Reeder, DeeAnn M.; Matuschewski, Kai; Schaer, JulianeHepatocystis parasites are the closest relatives of Plasmodium species of mammals. They infect monkeys, bats, squirrels, and ungulates in Africa, Asia, and Australia. A prevalence of up to 100% has been documented in fruit bats and monkeys. Twenty-five morphospecies have been described, and cross-species transmission, divergent Hepatocystis lineages, and species complexes are reported in primate and bat hosts. Biting midges (Culicoides) are the only known vectors. In the vertebrate, merogony occurs exclusively in the liver, resulting in formation of macroscopic merocysts. Merozoites invade erythrocytes and transform directly into sexual gametocytes, thereby omitting asexual replication and associated health conditions. Gametocytes can persist for several weeks and fertilize after a bloodmeal in the Culicoides midgut. The Hepatocystis genome features unique gene families, a low number of Plasmodium interspersed repeat (pir) genes, and an absence of the reticulocyte-binding protein family.Item Negative perception of bats, exacerbated by the SARS-CoV-2 pandemic, may Hhinder bat conservation in Northern Uganda(MDPI, 2022-12-16) Ejotre, Imran; Reeder, DeeAnn M; Matuschewski, Kai; Kityo, Robert; Schaer, JulianeBats face diverse challenges that cause global bat population declines, including habitat loss and roost disturbance. Additionally, negative perceptions of bats and their potential role in several zoonotic diseases have led to actions against bats. We documented existing knowledge and perception of bats through interviews with 151 participants of fifteen tribes in Northern Uganda in 2020 and conducted a sensitization campaign that prevented planned actions against bats. The interviews revealed distinct firm beliefs, negative perceptions, limited knowledge on bats, and the influence of media in shaping actions against bats. In addition, modified landscapes and habitat loss increased encounters and subsequent deterioration of relations between humans and bats. Targeted threats towards bats were exacerbated by public misinformation during the SARS-CoV-2 pandemic. No deliberate conservation efforts exist, and negative perception largely hampers the implementation of bat conservation in Northern Uganda. Importantly, the study also demonstrates that sensitization campaigns can be effective tools to protect bats in the short term. Regular sensitizations and education are recommended for sustainable changes in attitudes to and coexistence with bats.Item The diagnostic and prognostic value of exosomal microRNAs in lung cancer: a systematic review(Springer Nature, 2024-03-15) Yang, Bingbing; Xin, Xiaoqi; Cao, Xiaoqing; Nasifu, Lubanga; Nie, Zhenlin; He, BangshunStudies have shown that many exosomal microRNAs (miRNAs) can be used as non-invasive biomarkers of lung cancer, but their diagnostic and prognostic values need to be further clarified. We conducted a systematic literature search in Web of Science, PubMed, and ScienceDirect databases, obtained relevant articles and extracted data, and used statistical methods and statistical software to comprehensively evaluate the diagnostic and prognostic value of exosomal miRNAs in lung cancer. Registration number: PROSPERO CRD42023447398. In terms of diagnosis, two exosomal miRNAs (miR-486-5p and miR-451a) were reported with the highest frequency in lung cancer patients, both of which had good diagnostic value. Compared with the control group, the pooled sensitivities of miR-486-5p and miR-451a were 0.80 (95% CI: 0.73–0.86) and 0.76 (95% CI: 0.60–0.87), specificities: 0.93 (95% CI: 0.63–0.99) and 0.85 (95% CI: 0.72–0.92), and AUCs: 0.85 (95% CI: 0.81–0.88) and 0.88 (95% CI: 0.84–0.90), for the respective miRNAs. For prognosis, in lung cancer patients with abnormally expressed exosomal miRNAs, miR-1290 was associated with PFS outcome; miR-382, miR-1246, miR-23b-3p, miR-21-5p, and miR-10b-5p were associated with OS outcome; miR-21 and miR-4257 were associated with DFS outcome; miR-125a-3p and miR-625-5p were associated with PFS and OS outcomes; miR-216b and miR-451a were associated with OS and DFS outcomes. Exosomal miRNAs are valuable biomarkers in lung cancer patients. Exosomal miR-486-5p and miR-451a can be used as new diagnostic biomarkers for lung cancer. Dysregulated exosomal miRNAs could serve as indicators of survival outcomes in lung cancer patients.Item The correlation between equatorial electrojet and equatorial ionisation anomaly over the East African region during the solar minimum period 2008-2009(East African Journal Of Science, Technology and Innovation, 2023-09-29) Xiong, Mengqiu; Milimu, Hannington; Omondi, George; Mungufeni, PatrickThis study analyzed the correlation between the equatorial electrojet (EEJ) and the occurrence of equatorial ionisation anomaly (EIA) over the East African region. The study was carried out during both geomagnetically quiet and disturbed conditions when Kp index values were < 2+ and > 5+, respectively. The EEJ data were obtained using a pair of magnetometers located at Adis Ababa (geographic 9.04°N, 38.77°E, geomagnetic 0.17°N, 110.47°E) and Adigrat (geographic 14.281°N, 39.46°E, geomagnetic 6.0°N, 111.06°E), both in Ethiopia while the EIA data were derived from the total electron content (TEC) data that were obtained using a set of Global Navigation Satellite System (GNSS) receivers within the East African region. The data used were for the years 2008 and 2009. The TEC data over the crest of EIA were divided by those over the trough to quantify EIA strength over the region. The EEJ intensity was estimated from the difference in the horizontal component of the geomagnetic field observed by the pair of magnetometers. The results during quiet geomagnetic conditions showed that peak values of EEJ which range from 48nT - 110nT occurred between 10:00 and 14:00 LT. The EIA’s peak which varies from 1.20 to 1.45 occurs between 20:00 to 22:00 LT. The correlation coefficients were found to vary from moderate (0.58) to strongest (0.74). During geomagnetically disturbed conditions, the correlation coefficient ranges from 0.28 to 0.45. The increased eastward electric field and photo-ionization on TEC are responsible for the strong link between EEJ and EIA. This study reveals the trend in the variation of the strength of EEJ and EIA over the East African region which can be used as a basis for developing regional models to forecast or nowcast scintillations and the ionospheric space weather prediction over this region.Item Robust evidence for bats as reservoir hosts is lacking in most African virus studies: a review and call to optimize sampling and conserve bats(Royal Society, 2023-11-15) Weber, Natalie; Nagy, Martina; Markotter, Wanda; Schaer, Juliane; Puechmaille, Sébastien J.; Sutton, Jack; Dávalos, Liliana M.; Dusabe, Marie-Claire; Ejotre, Imran; Fenton, M. Brock; Knörnschild, Mirjam; López-Baucells, Adrià; Medellin, Rodrigo A.; Metz, Markus; Mubareka, Samira; Nsengimana, Olivier; O'Mara, M. Teague; Racey, Paul A.; Tuttle, Merlin; Twizeyimana, Innocent; Vicente-Santos, Amanda; Tschapka, Marco; Voigt, Christian C.; Wikelski, Martin; Dechmann, Dina K.N.; Reeder, DeeAnn M.Africa experiences frequent emerging disease outbreaks among humans, with bats often proposed as zoonotic pathogen hosts. We comprehensively reviewed virus–bat findings from papers published between 1978 and 2020 to evaluate the evidence that African bats are reservoir and/or bridging hosts for viruses that cause human disease. We present data from 162 papers (of 1322) with original findings on (1) numbers and species of bats sampled across bat families and the continent, (2) how bats were selected for study inclusion, (3) if bats were terminally sampled, (4) what types of ecological data, if any, were recorded and (5) which viruses were detected and with what methodology. We propose a scheme for evaluating presumed virus–host relationships by evidence type and quality, using the contrasting available evidence for Orthoebolavirus versus Orthomarburgvirus as an example. We review the wording in abstracts and discussions of all 162 papers, identifying key framing terms, how these refer to findings, and how they might contribute to people's beliefs about bats. We discuss the impact of scientific research communication on public perception and emphasize the need for strategies that minimize human–bat conflict and support bat conservation. Finally, we make recommendations for best practices that will improve virological study metadata.Item Assessing the quality of drinking water from selected water sources in Mbarara city, Southwestern Uganda(Plos one, 2024-03-28) Abaasa, Catherine N.; Ayesiga, Savino; Lejju, Julius B.; Andama, Morgan; Tamwesigire, Imelda K.; Bazira, Joel; Byarugaba, FrederickThis study assessed the physical, chemical, and microbiological quality with emphasis on risk score, source apportionment, geochemistry, feacal coliforms and water quality index of drinking water from selected water sources. A cross-sectional study was conducted in six villages in Mbarara city, south-western Uganda. Each selected source was inspected using a WHO-adopted sanitary inspection questionnaire. Each source’s risk score was calculated. Thirty-seven samples were taken from one borehole, nine open dug wells, four rain harvest tanks, and twenty-three taps. The values for apparent color and phosphate were higher than the permissible level as set by the World Health Organization and Ugandan standards (US EAS 12). The isolated organisms were Klebsiella spp. (8.11%), Citrobacter divergens (62.16%), Citrobacter fluendii (2.7%), E. coli (35.14%), Enterobacter aerogenes (8.11%), Enterobacter agglomerus (5.4%), Proteus spp. (2.7%), Enterobacter cloacae (13.5%), and Proteus mirabilis (2.7%). Twelve water sources (32.4%) had water that was unfit for human consumption that was unfit for human consumption (Grade E), Five sources (13.5%) had water that had a very poor index (Grade D), nine (24.3%) had water of poor index (Grade C), eight (21.6%) had water of good water index (Grade B), and only three (8.1%) had water of excellent water quality index (Grade A). The piper trilinear revealed that the dominant water type of the area were Mgso4 and Caso4 type. Gibbs plot represents precipitation dominance. PCA for source apportionment showed that well, tap and borehole water account for the highest variations in the quality of drinking water. These results suggest that drinking water from sources in Mbarara city is not suitable for direct human consumption without treatment. We recommend necessary improvements in water treatment, distribution, and maintenance of all the available water sources in Mbarara City, South Western Uganda.Item Agronomic suitability for oil palm growing in Uganda(Journal of Agricultural Science, 2024-03-15) Ddamulira, Gabriel; Asiimwe, Alex; Masika, Fred; Amugoli, Moses; Ddumba, Gerald; Maphosa, McebisiThe ever-increasing demand for vegetable oils with its products in Uganda, calls for new areas to be put under oil palm cultivation. A study was conducted to investigate the agronomic suitability of oil palm cultivation in six areas located within a 30 km radius off-shore, on the island and more than 30 km radius off-shore on the mainland. The experiment was superimposed on 45 × 45 m plots in oil palm adaptive trial plantations established in 2008 in Bugiri, Mayuge, Buvuma, Mukono, Kibaale and Masaka districts. The experiment was laid out in a randomized complete block design with three replications. Data on number of oil palm bunches, bunch weight and yield was collected while climatic data (rainfall, temperature, relative humidity and radiation) was acquired from meteorological stations near the experimental locations. Rainfall data analyzed and dry season duration suggested that Mayuge, Masaka, Mukono, Buvuma and Kibaale were moderately favorable for oil palm growing, while temperature and radiation data indicated that all study locations were suitable for oil palm growing. Furthermore, based on relative humidity, Buvuma, Mayuge and Bugiri were suitable for oil palm cultivation but Kibaale, Masaka and Mukono were moderately suitable. Oil palm yield varied significantly (P < 0.05) across locations. The yield was much greater in Mukono (17.7 t/ha/yr) followed by Buvuma (13.8 t/ha/yr) and Kibaale (12.9 t/ha/yr) then Mayuge (10.7 t/ha/yr), Bugiri (10.2 t/ha/yr), and Masaka (10.3 t/ha/yr). The significantly high yield of oil palm observed in Mukono was attributed to the high rainfall received in this location. Based on research findings, Mukono, Masaka, Bugiri, Buvuma, Kibaale and areas within the 30 km radius of Lake Victoria are agronomically suitable for oil palm cultivation.Item Ecological countermeasures to prevent pathogen spillover and subsequent pandemics(Springer Nature, 2024-03-26) Plowright, Raina K.; Ahmed, Aliyu N.; Coulson, Tim; Crowther, Thomas W.; Ejotre, Imran; Faust, Christina L.; Frick, Winifred F.; Hudson, Peter J.; Kingston, Tigga; Nameer, P. O.; O’Mara, M. Teague; Peel, AlisonJ.; Possingham, Hugh; Razgour, Orly; Reeder, DeeAnn M.; Ruiz-Aravena, Manuel; Simmons, Nancy B.; Srinivas, Prashanth N.; Tabor, Gary M.; Tanshi, Iroro; Thompson, Ian G.; Vanak, Abi T.; Vora, Neil M.; Willison, Charley E.; Keeley, Annika T. H.Substantial global attention is focused on how to reduce the risk of future pandemics. Reducing this risk requires investment in prevention, preparedness, and response. Although preparedness and response have received significant focus, prevention, especially the prevention of zoonotic spillover, remains largely absent from global conversations. This oversight is due in part to the lack of a clear definition of prevention and lack of guidance on how to achieve it. To address this gap, we elucidate the mechanisms linking environmental change and zoonotic spillover using spillover of viruses from bats as a case study. We identify ecological interventions that can disrupt these spillover mechanisms and propose policy frameworks for their implementation. Recognizing that pandemics originate in ecological systems, we advocate for integrating ecological approaches alongside biomedical approaches in a comprehensive and balanced pandemic prevention strategy.Item Two‑phase simulation of entropy optimized mixed convection flow of two different shear‑thinning nanomaterials in thermal and mass diffusion systems with Lorentz forces(Springer Nature, 2024-01-04) Suresha, S.; Khan, Umair; Soumya, D. O.; Venkatesh, P.; Gasmi, Hatem; Sunitha, M.; Zaib, Aurang; Al‑Naghi, Ahmed; Karoui, Hatem; Ishak, Anuar; Ojok, WalterThis research compares the momentum, thermal energy, mass diffusion and entropy generation of two shear thinning nanofluids in an angled micro-channel with mixed convection, nonlinear thermal radiation, temperature jump boundary condition and variable thermal conductivity effects. The approach was used to solve the Buongiorno nonlinear governing model. The effect of different parameters on the flow, energy, concentration, and entropy generating fields have been graphically illustrated and explained. The hyperbolic tangent nanoliquid has a better velocity than the Williamson nanofluid. The Williamson nanofluid has higher thermal energy and concentration than the hyperbolic tangent nanoliquid in the microchannel. The Grashof number, both thermal and solutal, increases the fluid flow rate throughout the flow system. The energy of the nanoliquid is reduced by the temperature jump condition, while the energy field of the nanoliquid is enhanced by the improving thermal conductivity value. The nanoliquids concentration rises as the Schmitt number rises. The irreversibility rate of the channel system is maximized by the variable thermal conductivity parameter.Item Predicting the thermal distribution in a convective wavy fin using a novel training physics-informed neural network method(Springer Nature, 2024-03-25) Chandan, K.; Saadeh, Rania; Qazza, Ahmad; Karthik, K.; Kumar, R. S. Varun; Kumar, R. Naveen; Khan, Umair; Masmoudi, Atef; Abdou, M. Modather M.; Ojok, Walter; Kumar, RamanFins are widely used in many industrial applications, including heat exchangers. They benefit from a relatively economical design cost, are lightweight, and are quite miniature. Thus, this study investigates the influence of a wavy fin structure subjected to convective effects with internal heat generation. The thermal distribution, considered a steady condition in one dimension, is described by a unique implementation of a physics-informed neural network (PINN) as part of machine-learning intelligent strategies for analyzing heat transfer in a convective wavy fin. This novel research explores the use of PINNs to examine the effect of the nonlinearity of temperature equation and boundary conditions by altering the hyperparameters of the architecture. The non-linear ordinary differential equation (ODE) involved with heat transfer is reduced into a dimensionless form utilizing the non-dimensional variables to simplify the problem. Furthermore, Runge–Kutta Fehlberg’s fourth–fifth order (RKF-45) approach is implemented to evaluate the simplified equations numerically. To predict the wavy fin's heat transfer properties, an advanced neural network model is created without using a traditional data-driven approach, the ability to solve ODEs explicitly by incorporating a mean squared error-based loss function. The obtained results divulge that an increase in the thermal conductivity variable upsurges the thermal distribution. In contrast, a decrease in temperature profile is caused due to the augmentation in the convective-conductive variable values.Item Genomic characterization of SARS-CoV-2 from Uganda using MinION nanopore sequencing(Springer Nature, 2023-11-22) Kia, Praiscillia; Katagirya, Eric; Kakembo, Fredrick Elishama; Adera, Doreen Ato; Nsubuga, Moses Luutu; Yiga, Fahim; Aloyo, Sharley Melissa; Aujat, Brendah Ronah; Anguyo, Denis Foe; Katabazi, Fred Ashaba; Kigozi, Edgar; Joloba, Moses L.; Kateete, David PatrickSARS-CoV-2 undergoes frequent mutations, affecting COVID-19 diagnostics, transmission and vaccine efficacy. Here, we describe the genetic diversity of 49 SARS-CoV-2 samples from Uganda, collected during the COVID-19 waves of 2020/2021. Overall, the samples were similar to previously reported SARS-CoV-2 from Uganda and the Democratic Republic of Congo (DRC). The main lineages were AY.46 and A.23, which are considered to be Delta SARS-CoV-2 variants. Further, a total of 268 unique single nucleotide variants and 1456 mutations were found, with more than seventy percent mutations in the ORF1ab and S genes. The most common mutations were 2042C>G (83.4%), 14143C>T (79.5%), 245T>C (65%), and 1129G>T (51%), which occurred in the S, ORF1ab, ORF7a and N genes, respectively. As well, 28 structural variants—21 insertions and 7 deletions, occurred in 16 samples. Our findings point to the possibility that most SARS-CoV-2 infections in Uganda at the time arose from local spread and were not newly imported. Moreover, the relatedness of variants from Uganda and the DRC reflects high human mobility and interaction between the two countries, which is peculiar to this region of the world.