Muni Repository (MR)
This repository contains open access publications of Muni University Library.
Objectives:
- To digitally collect, preserve and provide electronic access to scholarly works and research output of Muni University.
- Increase the visibility and impact of our research, making it easy for researchers, students, policymakers and journalists to reference, replicate, and re-use the work.
- Issue permanent, unique and trustworthy identifiers when creating URLs to access the resource without concern that the location of the resource may change.
How to publish in Muni Repository
- Contact the library through email: libsupport@muni.ac.ug
Communities in MR
Select a community to browse its collections.
Recent Submissions
Profiledistribution and health risk assessment of per- and polyfluoroalkyl substances in indoor dust from urban households in Uganda, East Africa
(Elsevier, 2026-02-03)
Per- and polyfluoroalkyl substances (PFAS) contamination of various environmental compartments continues to attract scrutiny due to their persistence, bioaccumulation and toxicity, following their widespread application over the years as surfactants and stain repellents. Studies have indicated that environments, including dust, may serve as sources of human exposure. However, little is known about the prevalence of PFAS and their associated health risks in indoor environments from the sub-Saharan African countries, including Uganda. Herein, we collected samples of indoor household dust from the floors of residential houses (n = 43) in Kampala, Uganda, to assess the levels and associated health risks of PFAS to the exposed humans. Of the 11 targeted PFAS, only 8 were detected in the collected samples. The mean level of ∑8PFAS in household dust was 427 ng/g, with a maximum concentration of 2850 ng/g. 10:2 fluorotelomer alcohol (10:2 FTOH), and perfluorobutanoic acid (PFBA) predominated among the neutral and ionic PFAS, respectively. Source apportionment revealed food packaging, semiconductor materials and textiles as the major sources. The calculated hazard quotients (HQs) due to exposure to PFAS, and their sums (∑HQs) in our study for both adults and toddlers, were all less than 1, suggesting negligible non-carcinogenic risk. Despite the low estimated exposure via dust ingestion and dermal contact, compound-specific toxicological profiles of PFAS, their potential for interactive effects, and the presence of additional exposure routes underscore the urgency of regularly investigating indoor PFAS-related health risks.
Exposure of urban population to organophosphate esters and novel brominated flame retardants via indoor dust: Occurrence, sources and health risks in Uganda, East Africa
(Elsevier, 2026-01-29)
Indoor environments are critical exposure pathways to flame retardants, yet data from Africa remain scarce. This study provides the first quantitative assessment of organophosphate esters (OPEs) and novel brominated flame retardants (NBFRs) in indoor dust from Ugandan households, establishing baseline data for East Africa. Dust samples collected from homes in Kampala were analyzed using gas chromatography–mass spectrometry (GC–MS). Concentrations of total OPEs (Σ5OPEs) and total NBFRs (Σ4NBFRs) ranged from 1520 to 102,000 ng g−1 and 214 to 6600 ng g−1, respectively. Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) and 2,4,6-tribromophenyl allyl ether (TBP-AE) dominated OPE and NBFR profiles, respectively. Chlorinated OPEs contributed most to ΣOPEs, reflecting emissions from building materials and consumer products. Principal component analysis indicated household items, electrical equipment, and floor finishes as the major emission sources. Estimated human exposure doses showed that ingestion was the dominant route, with children more exposed than adults. Hazard index (HI) values were below 1 for all compounds, indicating negligible non-carcinogenic risk. Carcinogenic risk (CR) values ranged from 10−6 to 10−12, suggesting minimal lifetime cancer risks, though children exhibited relatively higher vulnerability. Compared with global datasets, OPE and NBFR levels in Kampala were within lower-to-median range but represent the inaugural dataset for sub-Saharan Africa. These findings provide a crucial regional reference for indoor flame-retardant exposure and highlight the need for extended studies across multiple indoor microenvironments to evaluate the long-term exposure pathways and inform safer chemical management.
Spent Pleurotus ostreatus Substrate Has Potential for Controlling the Plant-Parasitic Nematode, Radopholus similis in Bananas
(MDPI, 2025-04-26) Tazuba, Anthony Fredrick; Ocimati, Walter; Ogwal, Geofrey; Nyangwire, Betty; Onyilo, Francis; Blomme, Guy
Spent mushroom substrate (SMS), a waste product from mushroom cultivation, in addition to being rich in essential nutrients for crop growth, contains actively growing mushroom mycelia and metabolites that suppress some plant pathogens and pests. SMS thus has potential for fostering the suppressiveness of soil-borne pathogens of farms. This study determined the potential of using the spent Pleurotus ostreatus substrate (SPoS) to suppress the plant-parasitic nematode Radopholus similis in bananas. R. similis is the most economically important nematode in bananas worldwide. The effect of SPoS on R. similis was assessed through two in vivo (potted plants) experiments between May 2023 and June 2024. Five-month-old East African highland banana (genome AAA) plantlets that are highly susceptible to R. similis were used. In the first experiment, the plantlets were established in 3 L pots containing (i) pre-sterilized soil, (ii) pre-sterilized soil inoculated with nematodes, (iii) pre-sterilized soil mixed with 30% (v/v) SPoS, (iv) pre-sterilized soil mixed with 30% (v/v) SPoS followed by nematode inoculation, (v) SPoS without soil, and (vi) SPoS without soil inoculated with nematodes. The SPoS was already decomposed; thus, it may or may not have contained active mycelia. The nematodes were introduced two weeks after the SPoS application. In the second experiment, SPoS was introduced two weeks after nematode inoculation. The SPoS treatments without soil were not evaluated in the second experiment. Both experiments were monitored over a three-month period. Each screenhouse treatment contained four plants and was replicated thrice. In the first experiment, data were collected on changes in soil nutrient content, below- and aboveground biomass, root deaths, root necrosis due to nematode damage, and R. similis population in root tissues and soil. In the second experiment, data were collected on root deaths and the number of nematodes in root tissues and the soil. The SPoS improved crop biomass yield, reduced root damage, and colonization by R. similis. The potential of SPoS to improve the management of R. similis and banana production under field conditions needs to be determined.
Pleurotus ostreatus is a potential biological control agent of root-knot nematodes in eggplant (Solanum melongena)
(Frontiers Media, 2024-10-25) Nyangwire, Betty; Ocimati, Walter; Tazuba, Anthony Fredrick; Blomme, Guy; Alumai, Alfred; Onyilo, Francis
Introduction: The management of root-knot nematodes has predominantly been based on use of chemicals, which are detrimental to the environment and human health. Biological control provides alternative management. This study evaluated the potential of using Pleurotus ostreatus, an edible mushroom species to control Meloidogyne spp. in eggplants.
Methods: In vitro, the mortality of juveniles (J2) of Meloidogyne spp. were assessed i) P. ostreatus - water suspension with actively growing mycelia, and ii) different dilutions of P. ostreatus PDB broth culture filtrates. In the screen house nematicidal potential of P. ostreatus was tested on eggplants using artificially inoculated soils in a screen house. To attain this, juveniles (J2) of Meloidogyne spp. were inoculated at the base of plants in pots containing P. ostreatus colonized millet grains mixed in 3 kg of soil. The galling index (GI) (scale of 0 to 5), root growth and nematode populations in the different treatments were assessed.
Results and discussion: Mortality of nematodes in the P. ostreatus - water suspension significantly increased with time, reaching over 88% at 48 h and 95% at 72 h. Mortality in undiluted filtrate was consistently significantly higher than the diluted filtrates and control without P. ostreatus. Mortality in the undiluted filtrate increased to 95% at 48 h. When 50 g of P. ostreatus-millet culture was mixed with 3 kg autoclaved pot soil, a GI of 0.95 was observed, dropping to 0.70 when the inoculum was doubled to 100 g. For the treatment without P. ostreatus, a high GI of 2.4 was scored. A significant difference in eggplant root growth and nematode population at (p = 0.02) was found across the treatments. The findings from this study for both in-vitro and pot assay suggest that P. ostreatus and its substrate are potential biological control agents for plant parasitic nematodes in eggplants.
Tuberculosis-related stigma and associated factors among people with pulmonary tuberculosis predominantly co-infected with HIV in Kampala, Uganda: a cross-sectional study
(Elsevier, 2026-03-18) Izudi, Jonathan; Kyazze, Simon; Bajunirwe, Francis
Rationale
Stigma has a profound effect on the well-being and treatment outcomes of people with tuberculosis (PWTB). Despite its negative effects, TB-related stigma remains underexplored in high-burden TB settings such as Uganda.
Objective
To examine the factors associated with tuberculosis (TB)-related stigma among people with drug-susceptible pulmonary TB aged ≥18 years in Kampala, Uganda.
Methods
We conducted a cross-sectional study across five primary health facilities. The primary exposure was psychological well-being and was assessed using the World Health Organization (WHO) Five Well-Being Index (WHO-5). Scores ranged from 0 to 20, with ≥15 indicating good psychological well-being, and <15 indicating poor psychological well-being. TB-related stigma was the primary outcome measured using the Van Rie Stigma Scale, ranging from 0 to 48. We applied Generalized Estimating Equations, adjusting for important covariates and clustering by health facility to determine associated factors. Beta coefficients (β) and 95% confidence intervals (CI) were reported.
Results
We analyzed data from 818 participants, with normally distributed TB-related stigma scores: 25.3 ± 6.45. Higher TB-related stigma scores were statistically significantly associated with poor psychological well-being (β = 0.86, 95% CI: 0.60–1.13) and being a male (β = 0.77, 95% CI: 0.53–1.00). Individuals aged ≥25 years showed a borderline statistically significant association with TB-related stigma (β = 0.83, 95% CI: 0.11–1.55).
Conclusion
This study showed that poor psychological well-being and being male are associated with higher TB-related stigma scores among PWTB in Kampala, Uganda. TB programs should integrate mental health and implement stigma-reduction strategies that address underlying causes.