Rapid detection of Helicobacter pylori and its virulence genes by fluorescence-chromogenic double-indicator LAMP (FC-LAMP)

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dc.contributor.authorLiu, Yanghe
dc.contributor.authorYin, Sijie
dc.contributor.authorChen, Shiyue
dc.contributor.authorZhang, Chunlei
dc.contributor.authorLubanga, Nasifu
dc.contributor.authorTai, Ping
dc.contributor.authorXiong, Mengqiu
dc.contributor.authorFan, Boyue
dc.contributor.authorYang, Xincheng
dc.contributor.authorXia, Xinyi
dc.contributor.authorHou, Panfei
dc.contributor.authorHe, Bangshun
dc.date.accessioned2025-11-25T09:51:18Z
dc.date.available2025-11-25T09:51:18Z
dc.date.issued2025-10-15
dc.description.abstractHelicobacter pylori (H. pylori) is a risk factor of gastrointestinal diseases. Vacuolating cytotoxin A (VacA) is a key component of the pathogenicity of H. pylori for gastric diseases, and detection for virulence genotypes facilitates precise clinical diagnosis and individual treatment. Hence, a more accurate, convenient, and highly sensitive detection method is urgently needed. In this study, we developed a method based on fluorescence-colorimetric dual-indicator loop-mediated isothermal amplification (FC-LAMP), allowing for naked-eye readout, using a constant temperature water bath within 40 min in clinical settings. Such an excellent sensitivity and specificity, and user-friendly operation approach achieved a limit of 10−6 ng/μl for 16 s rRNA detection of H. pylori, and 100 % specificity for 55 clinical gastric fluid samples compared with the immunofluorescence staining (IFS) and quantitative polymerase chain reaction (qPCR) as reference methods. In short, this study offers a new strategy for detection of H. pylori and its virulence genotype in primary hospitals and in limited resources settings, facilitating individualized precision eradication of H. pylori.
dc.description.sponsorship2024 Nanjing Jiangning District Science and Technology Benefit People Plan Project(2024045S); The research project of Jiangsu Health Development Research Center (JSHD2022045); Natural Science Foundation of Jiangsu Province (BK20240257);Research Project Funding from Affiliated Jiangning Hospital of Chinese Medicine, China Pharmaceutical University (YJQN2025008); Jiangsu Provincial Medical Key Discipline Cultivation Unit (JSDW202239), Nanjing Medical Key Laboratory of Laboratory Diagnostics.
dc.identifier.citationLiu, Y., Yin, S., Chen, S., Zhang, C., Lubanga, N., Tai, P., Xiong, M., Fan, B., Yang, X., & Xia, X. (2025). Rapid detection of Helicobacter pylori and its virulence genes by fluorescence-chromogenic double-indicator LAMP (FC-LAMP). Clinica Chimica Acta, 120667.
dc.identifier.issn0009-8981
dc.identifier.urihttps://dir.muni.ac.ug/handle/20.500.12260/808
dc.language.isoen
dc.publisherElsevier
dc.subjectHelicobacter pylori
dc.subjectLoop-mediated isothermal amplification
dc.subjectFluorescence-colorimetric dual-indicator
dc.subjectVirulence genes
dc.titleRapid detection of Helicobacter pylori and its virulence genes by fluorescence-chromogenic double-indicator LAMP (FC-LAMP)
dc.typeArticle

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