Rapid detection of Helicobacter pylori and its virulence genes by fluorescence-chromogenic double-indicator LAMP (FC-LAMP)
Loading...
Date
2025-10-15
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Helicobacter pylori (H. pylori) is a risk factor of gastrointestinal diseases. Vacuolating cytotoxin A (VacA) is a key component of the pathogenicity of H. pylori for gastric diseases, and detection for virulence genotypes facilitates precise clinical diagnosis and individual treatment. Hence, a more accurate, convenient, and highly sensitive detection method is urgently needed. In this study, we developed a method based on fluorescence-colorimetric dual-indicator loop-mediated isothermal amplification (FC-LAMP), allowing for naked-eye readout, using a constant temperature water bath within 40 min in clinical settings. Such an excellent sensitivity and specificity, and user-friendly operation approach achieved a limit of 10−6 ng/μl for 16 s rRNA detection of H. pylori, and 100 % specificity for 55 clinical gastric fluid samples compared with the immunofluorescence staining (IFS) and quantitative polymerase chain reaction (qPCR) as reference methods. In short, this study offers a new strategy for detection of H. pylori and its virulence genotype in primary hospitals and in limited resources settings, facilitating individualized precision eradication of H. pylori.
Description
Keywords
Helicobacter pylori, Loop-mediated isothermal amplification, Fluorescence-colorimetric dual-indicator, Virulence genes
Citation
Liu, Y., Yin, S., Chen, S., Zhang, C., Lubanga, N., Tai, P., Xiong, M., Fan, B., Yang, X., & Xia, X. (2025). Rapid detection of Helicobacter pylori and its virulence genes by fluorescence-chromogenic double-indicator LAMP (FC-LAMP). Clinica Chimica Acta, 120667.