In vitro antifungal activities of medicinal plants used for treatment of candidiasis in Pader district, Northern Uganda

Simple item page
dc.contributor.authorAkwongo, Betty
dc.contributor.authorKakudidi, Esezah K.
dc.contributor.authorNsubuga, Anthony M.
dc.contributor.authorAndama, Morgan
dc.contributor.authorNamaganda, Mary
dc.contributor.authorTugume, Patience
dc.contributor.authorAsiimwe, Savina
dc.contributor.authorAnywar, Godwin
dc.contributor.authorKatuura, Esther
dc.date.accessioned2024-11-14T06:32:48Z
dc.date.available2024-11-14T06:32:48Z
dc.date.issued2024-11-13
dc.description.abstractBackground: The emergence of multidrug resistant Candida species to available drugs has led to renewed interest in the use of herbal medicines globally. This study scientifically verified antifungal effectiveness of five commonly used plant species in Pader district, against selected pathogenic candida strains. Methods: Powdered roots of Momordica foetida, Sansevieria dawei and Distimake dissectus; and stem barks of Khaya anthotheca and Mitragyna rubrostipulata were extracted sequentially using petroleum ether and methanol, respectively; and total water extraction at 24.4 °C (maceration), 60 °C (decoction) and boiling water at 87 °C (hot water infusion). Extracts and their combinations, positive controls (amphotericin B, and fluconazole) and negative control (80% dimethyl sulfoxide, verified to be tolerable concentration to the tested Candida species) were screened and verified for their antifungal activity against Candida albicans (ATCC: American Type Culture Collection reference strain 10231, ATCC 90028, 0770a and 0796), C. glabrata (VVc 004, ATCC 2950) and C. tropicalis (ATCC 750 and 0210) using agar well diffusion and broth micro-dilution, respectively. Results: Aqueous extract (24.4 °C) of M. rubrostipulata (ZOI: 18.00 ± 1.00 to 38.33 ± 0.17; MIC: 3.13 ± 0.00 to 20.83 ± 4.17; MFC: 12.50 ± 0.00 to 200.00 ± 0.00), methanol extract of K. anthotheca (10.11 ± 0.31 to 15.11 ± 0.65; 1.04 ± 0.26 to 12.50 ± 0.00; 12.50 ± 0.00 to 100.00 ± 0.00), and combination of aqueous extract (60 °C) of D. dissectus + methanol extract of K. anthotheca (7.89 ± 0.26 to 19.67 ± 0.37; 0.78 ± 0.00 to 50.00 ± 0.00; 12.50 ± 0.00 to 200.00 ± 0.00) exhibited broad spectrum antifungal activities and were fungistatic against all tested Candida species, which comprised 8 clinical/control and susceptible/resistant strains. None of the conventional drugs used demonstrated broad spectrum antifungal activity across all tested Candida species/strains. Conclusion: Methanol extract of K. anthotheca, aqueous extract (24.4 °C) of M. rubrostipulata, and combination of aqueous extract (60 °C) of D. dissectus + methanol extract of K. anthotheca could be effective in the treatment of candidiasis. They demonstrated potential broad spectrum antifungal activity against different species and strains of tested Candida than the fluconazole and amphotericin B drugs. Their fungistatic nature showed their ability to inhibit fungal growth. Hence, these extracts/extract combination can offer better treatment option for candidiasis if they are standardized and also their active curative compounds isolated and made into antifungal drugs.
dc.description.sponsorshipMakerere University PhD Research and Innovation Fund (MAKRIF) Round 4, 2022/23
dc.identifier.citationAkwongo, B., Kakudidi, E. K., Nsubuga, A. M., Andama, M., Namaganda, M., Tugume, P., Asiimwe, S., Anywar, G., & Katuura, E. (2024). In vitro antifungal activities of medicinal plants used for treatment of candidiasis in Pader district, Northern Uganda. Tropical Medicine and Health, 52(1), 84. https://doi.org/10.1186/s41182-024-00628-x
dc.identifier.issn1349-4147
dc.identifier.urihttps://dir.muni.ac.ug/handle/20.500.12260/698
dc.language.isoen
dc.publisherSpringer Nature
dc.subjectMedicinal plants
dc.subjectAntifungal activity
dc.subjectCandidiasis
dc.subjectCandida species
dc.subjectMultidrug resistance
dc.subjectMinimum inhibitory concentration (MIC)
dc.subjectMinimum fungicidal concentration (MFC)
dc.titleIn vitro antifungal activities of medicinal plants used for treatment of candidiasis in Pader district, Northern Uganda
dc.typeArticle

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Akwongo_Article_2024_1.pdf
Size:
1.41 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.17 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles