Two new compounds and the anti-mycobacterial activity of the constituents from Zanthoxylum leprieurii root bark

dc.contributor.authorOloya, Benson
dc.contributor.authorNamukobe, Jane
dc.contributor.authorHeydenreich, Matthias
dc.contributor.authorSsengooba, Willy
dc.contributor.authorMartin, Johannes
dc.contributor.authorM¨oller, Heiko Michael
dc.contributor.authorSchmidt, Bernd
dc.contributor.authorByamukama, Robert
dc.date.accessioned2023-02-23T13:28:12Z
dc.date.available2023-02-23T13:28:12Z
dc.date.issued2023-02-08
dc.description.abstractThe emergence of resistance to the existing TB drugs necessitated a search for new anti-mycobacterial compounds from Z. leprieurii root bark, which is used locally for the treatment of tuberculosis (TB) in Uganda. Two new compounds: (E)-N-isobutyl-3-(4-((3-methylbut-2-en-1-yl)oxy)phenyl)acrylamide (1) and 4-(5,5a,6,8,8a,9-hexahydrofuro[3′,4′:6,7]-naphtha[2,3-d][1,3]dioxol-5-yl)-2-methoxyphenol (2), were isolated from the dichloromethane (DCM) extract. In addition, sixteen known compounds were isolated from the DCM and methanol extracts, with two of them, N-isobutylcinnamamide (9) and (E)-3-(4-hydroxy-3-methoxyphenyl)-N-isobutylacrylamide (14), being reported from a natural source for the first time. The structures of the isolates were elucidated by a combination of spectroscopic techniques. The isolated compounds were subjected to anti-mycobacterial activity testing using the microplate Alamar blue assay (MABA). Five compounds, 10 (MIC, 0.98 μg/mL), 11 (MIC, 7.82 μg/mL), 12 (MIC, 1.95 μg/mL), 13 (MIC, 3.91 μg/mL), and 14 (MIC, 3.91 μg/mL) exhibited significant anti-mycobacterial activities against the susceptible (H37Rv) strain and could provide vital templates for developing new and highly effective TB drugs. Also, compounds 4 (MIC, 62.50 μg/mL), 7 (MIC, 62.50 μg/mL) and 16 (MIC, 62.50 μg/mL) exhibited moderate activity against H37Rv.en_US
dc.description.sponsorshipOrganization for the Prohibition of Chemical Weapons (OPCW)en_US
dc.identifier.citationOloya, B., Namukobe, J., Heydenreich, M., Ssengooba, W., Martin, J., Möller, H. M., ... & Byamukama, R. (2023). Two new compounds and the anti-mycobacterial activity of the constituents from Zanthoxylum leprieurii root bark. Phytochemistry Letters, 54, 107-113.en_US
dc.identifier.issn1874-3900
dc.identifier.urihttps://doi.org/10.1016/j.phytol.2023.02.002
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.subjectAnti-mycobacterial activityen_US
dc.subjectZanthoxylum leprieuriien_US
dc.subjectTuberculosisen_US
dc.subjectAdubangoamideen_US
dc.subjectDranzoanolen_US
dc.titleTwo new compounds and the anti-mycobacterial activity of the constituents from Zanthoxylum leprieurii root barken_US
dc.typeArticleen_US

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