Extracellular hydrolytic enzyme activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus with oropharyngeal candidiasis at HIV/AIDS clinics in Uganda
| dc.contributor.author | Musinguzi, Benson | |
| dc.contributor.author | Akampurira, Andrew | |
| dc.contributor.author | Derick, Hope | |
| dc.contributor.author | Turyamuhika, Laban | |
| dc.contributor.author | Mwesigwa, Alex | |
| dc.contributor.author | Mwebesa, Edson | |
| dc.contributor.author | Mwesigye, Vicent | |
| dc.contributor.author | Kabajulizi, Immaculate | |
| dc.contributor.author | Sekulima, Tahalu | |
| dc.contributor.author | Ocheng, Francis | |
| dc.contributor.author | Itabangi, Herbert | |
| dc.contributor.author | Mboowa, Gerald | |
| dc.contributor.author | Sande, Obondo James | |
| dc.contributor.author | Achan, Beatrice | |
| dc.date.accessioned | 2024-12-20T13:34:58Z | |
| dc.date.available | 2024-12-20T13:34:58Z | |
| dc.date.issued | 2024-12-14 | |
| dc.description.abstract | Background: Commensal oral Candida species can become opportunistic and transition to pathogenic causes of oropharyngeal candidiasis (OPC) in individuals with impaired immunity through ecological cues and the expression of extracellular hydrolytic enzyme activities and biofilm formation. Objective: We evaluated phospholipase, proteinase, hemolysin, esterase, and coagulase enzymatic activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus (PLHIV) with OPC. Methods: Thirty-five Candida isolates from PLHIV with OPC were retrieved from a sample repository and evaluated for phospholipase activity using the egg yolk agar method, proteinase activity using the bovine serum albumin agar method, hemolysin activity using the blood agar plate method, esterase activity using the Tween 80 opacity test medium method, coagulase activity using the classical tube method, and biofilm formation using the microtiter plate assay method in vitro. Results: A total of 35 Candida isolates obtained from PLHIV with OPC were included in this study, and phospholipase and proteinase activities were detected in 33/35 (94.3 %) and 31/35 (88.6 %) Candida isolates, respectively. Up to 25/35 (71.4 %) of the Candida isolates exhibited biofilm formation, whereas esterase activity was demonstrated in 23/35 (65.7 %) of the Candida isolates. Fewer isolates (21/35, 60 %) produced hemolysin, and coagulase production was the least common virulence activity detected in 18/35 (51.4 %) of the Candida isolates. Conclusion: Phospholipase and proteinase activities were the strongest in oropharyngeal Candida species. | |
| dc.description.sponsorship | This study was funded by the government of Uganda through the Makerere University Research and Innovation Fund (MakRIF 5, 2023–2024) and Muni University Research and Innovation Fund (MRIF 02/06). Beatrice Achan is a fellow of NURTURE (NIH/International Fogarty Centre) under the NIH grants D43TW010132, EU EDCTP (TMA2018CDF-2371) | |
| dc.identifier.citation | Musinguzi, B., Akampurira, A., Derick, H., Turyamuhika, L., Mwesigwa, A., Mwebesa, E., ... & Achan, B. (2024). Extracellular Hydrolytic Enzyme Activities and Biofilm Formation in Candida species Isolated from People Living with Human Immunodeficiency Virus with Oropharyngeal Candidiasis at HIV/AIDS Clinics in Uganda. Microbial Pathogenesis, 107232. | |
| dc.identifier.issn | 1096-1208 | |
| dc.identifier.uri | https://dir.muni.ac.ug/handle/20.500.12260/709 | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.subject | Phospholipase | |
| dc.subject | Proteinase | |
| dc.subject | Hemolysin | |
| dc.subject | Esterase | |
| dc.subject | Coagulase | |
| dc.subject | Biofilm | |
| dc.title | Extracellular hydrolytic enzyme activities and biofilm formation in Candida species isolated from people living with human immunodeficiency virus with oropharyngeal candidiasis at HIV/AIDS clinics in Uganda | |
| dc.type | Article |