Molecular characterization of extended-spectrum beta-lactamase- producing bacteria isolated from pregnant women’s urine at Itojo Hospital, South Western Uganda

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dc.contributor.authorTwinomujuni, Muzafaru
dc.contributor.authorMusinguzi, Benson
dc.contributor.authorAsiimwe, Moses
dc.contributor.authorMpiima, Stephen Samuel
dc.contributor.authorZamarano, Henry
dc.contributor.authorOrikushaba, Isaac
dc.contributor.authorMuhanguzi, Deus
dc.contributor.authorTwinamatsiko, Crinad
dc.contributor.authorMallya, Sarapia Paul
dc.contributor.authorSamiri, Jamiru
dc.contributor.authorKamugisha, Joseph
dc.contributor.authorNalumaga, Pauline Petra
dc.contributor.authorKabanda, Taseera
dc.contributor.authorKassaza, Kennedy
dc.contributor.authorBagenda, Charles Nkubi
dc.contributor.authorTuhamize, Barbra
dc.contributor.authorBazira, Joel
dc.contributor.authorRicciardelli, Rosemary
dc.contributor.authorMpeirwe, Moses
dc.date.accessioned2026-03-23T17:55:28Z
dc.date.available2026-03-23T17:55:28Z
dc.date.issued2026-03-11
dc.descriptionThis study demonstrates the importance of addressing ESBL-producing bacteria among pregnant women by providing essential evidence to guide treatment and control strategies for antimicrobial resistance. The findings directly support SDG 3 (Good Health and Well-being), align with the Uganda National Development Plan IV, and advance improved maternal health, prevention efforts, and health system efficiency. The research underpins antimicrobial stewardship, informs clinical decision-making, and guides policies to enhance healthcare quality and lower infection risks in Uganda.
dc.description.abstractBackground: Extended-spectrum β-lactamase (ESBL)-producing bacteria pose a global challenge because of resistance developing against a wide range of antimicrobial agents, complicating available treatment options. Thus, identifying the prevalent bacterial species producing ESBL enzymes and understanding how they are susceptible to antibiotics is necessary to inform effective treatment guidelines. Objective: We sought to characterize ESBL-producing bacteria isolated from pregnant women’s urine at Itojo Hospital, Ntungamo district, Southwestern Uganda. Methods: We conducted a cross-sectional study where we collected and analysed 340 urine samples from 340 pregnant women. We did antimicrobial susceptibility testing using the Kirby–Bauer disc diffusion method. Isolates were screened for ESBL production and confirmed using the combination disc test. Genotypic characterization was confirmed using multiplex PCR to detect blaTEM, blaCTX-M and blaSHV genes. Results: The prevalence of ESBL-producing bacteria was 29.7% (101/340). Escherichia coli 36/101 (35.6%) and Klebsiella species 33/101 (32.7%) were predominant ESBL producers. Genotypic analysis revealed blaTEM 50/101 (49.5%) and blaCTX-M 31/101 (30.7%) as the most prevalent genes, while blaSHV was less common, 8/101 (7.9%) Conclusion. The high prevalence of ESBL-producing bacteria and their resistance to commonly used antibiotics highlighted the need for targeted antibiotic therapy, antimicrobial stewardship and regular molecular surveillance.
dc.identifier.citationTwinomujuni, M., Musinguzi, B., Asiimwe, M., Mpiima, S. S., Zamarano, H., Orikushaba, I., ... & Mpeirwe, M. (2026). Molecular characterization of extended-spectrum beta-lactamase-producing bacteria isolated from pregnant women’s urine at Itojo Hospital, South Western Uganda. Access Microbiology, 8(3), 001045-v5.
dc.identifier.issn2516-8290
dc.identifier.urihttps://dir.muni.ac.ug/handle/20.500.12260/942
dc.language.isoen
dc.publisherMicrobiology Society
dc.subjectAntimicrobial
dc.subjectAntenatal clinic
dc.subjectExtended spectrum β-lactamase
dc.subjectPregnancy
dc.titleMolecular characterization of extended-spectrum beta-lactamase- producing bacteria isolated from pregnant women’s urine at Itojo Hospital, South Western Uganda
dc.typeArticle

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