Browsing by Author "Opoke, Robert"

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    An ethnobotanical survey of plant species used for medicinal purposes in Amuru district, northern Uganda
    (Ilia State University, 2024-09-12) Gum, Bornfree; Opoke, Robert; Akwongo, Betty; Oloya, Benson; Omony, John Bosco; Opiro, Robert; Andama, Morgan; Anywar, Godwin; Malinga, Geoffrey Maxwell
    Background: Medicinal plants provide health care to rural communities that have limited access to modern medicine in Uganda. Thus, documenting medicinal plants is important for their sustainable utilisation and conservation of medicinal plants and associated indigenous knowledge. Methods: A cross-sectional ethnobotanical survey was conducted in August 2021 to assess the knowledge and use of medicinal plants in Lamogi Sub-County, Amuru district. We randomly sampled and interviewed 334 household heads using semi-structured questionnaires. Data was analysed using descriptive statistics, familiarity index (FI), fidelity level (FL) and informant consensus factor (ICF). Results: Most of the respondents (90%) had knowledge of medicinal plants used for treating ailments in their community (high ICF values above 0.9). Plant with the highest FI value was Gymnanthemum amygdalinum (Delile) Sch.Bip. indicating its wide use in the community. Chi-square tests showed significant associations between the knowledge of medicinal plants and education level, occupation, age, marital status and monthly income of respondents (p < 0.05). Forty-nine medicinal plants from 30 families were documented, with Fabaceae and Asteraceae having the most species. Leaves were the most frequently used plant parts (65.4%), followed by roots (13.6%). Herbal remedies were mostly prepared by crushing and smearing on the body, infusion, decoction and administered orally. Conclusion: The people of Amuru district have rich indigenous knowledge of diverse medicinal plants used for treating ailments. Most medicinal plants are harvested from the wild, threatening their survival and this requires putting in place practical conservation measures.
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    Apparent density, trypanosome infection rates and host preference of tsetse flies in the sleeping sickness endemic focus of Northwestern Uganda.
    (Springer Nature, 2021-11-29) Opiro, Robert; Opoke, Robert; Angwech, Harriet; Nakafu, Esther; Oloya, Francis A; Openy, Geoffrey; Njahira, Moses; Macharia, Mercy; Echodu, Richard; Malinga, Geoffrey M.; Opiyo, Elizabeth A.
    African trypanosomiasis, caused by protozoa of the genus Trypanosoma and transmitted by the tsetse fly, is a serious parasitic disease of humans and animals. Reliable data on the vector distribution, feeding preference and the trypanosome species they carry is pertinent to planning sustainable control strategies. We deployed 109 biconical traps in 10 villages in two districts of northwestern Uganda to obtain information on the apparent density, trypanosome infection status and blood meal sources of tsetse flies. A subset (272) of the collected samples was analyzed for detection of trypanosomes species and sub-species using a nested PCR protocol based on primers amplifying the Internal Transcribed Spacer (ITS) region of ribosomal DNA. 34 blood-engorged adult tsetse midguts were analyzed for blood meal sources by sequencing of the mitochondrial cytochrome c oxidase 1 (COI) and cytochrome b (cytb) genes. We captured a total of 622 Glossina fuscipes fuscipes tsetse flies (269 males and 353 females) in the two districts with apparent density (AD) ranging from 0.6 to 3.7 flies/trap/day (FTD). 10.7% (29/272) of the flies were infected with one or more trypanosome species. Infection rate was not significantly associated with district of origin (Generalized linear model (GLM), χ2 = 0.018, P = 0.895, df = 1, n = 272) and sex of the fly (χ2 = 1.723, P = 0.189, df = 1, n = 272). However, trypanosome infection was highly significantly associated with the fly’s age based on wing fray category (χ2 = 22.374, P < 0.001, df = 1, n = 272), being higher among the very old than the young tsetse. Nested PCR revealed several species of trypanosomes: T. vivax (6.62%), T. congolense (2.57%), T. brucei and T. simiae each at 0.73%. Blood meal analyses revealed five principal vertebrate hosts, namely, cattle (Bos taurus), humans (Homo sapiens), Nile monitor lizard (Varanus niloticus), African mud turtle (Pelusios chapini) and the African Savanna elephant (Loxodonta africana). We found an infection rate of 10.8% in the tsetse sampled, with all infections attributed to trypanosome species that are causative agents for AAT. However, more verification of this finding using large-scale passive and active screening of human and tsetse samples should be done. Cattle and humans appear to be the most important tsetse hosts in the region and should be considered in the design of control interventions.
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    An ethnobotanical study of plants used for the treatment of malaria in Budondo sub-county, Eastern Uganda
    (Ethnobotany Research and Applications, 2020-01-30) Malinga, M. Geoffrey; Baana, Kalori; Rutaro, Karlmax; Atube, Francis; Opoke, Robert; Opika-Opoka, Hector; Oryema, Christine
    Background: Malaria is a leading cause of morbidity and mortality in most developing countries, and in Uganda over 95% of the country is endemic with malaria. Given the increasing widespread resistance to current drugs, the high cost of procurement of antimalarial drug combination therapy, the use of herbal medicines is seen as a sustainable solution to malaria treatment. This study documented medicinal plants that are traditionally used for the treatment of malaria in Budondo sub-county, Eastern Uganda. Methods: The ethnobotanical survey was conducted between December 2017 to January 2018. A total of 273 household members were interviewed on knowledge and use of anti-malarial plants, using semi-structured questionnaires administered in five parishes of Budondo sub-county. Voucher specimen of each plant species were preserved at the Makerere University herbarium, Uganda. Results: Overall, 97.5% of the respondents had adequate knowledge regarding the plants used to treat malaria. A chi-square analysis shows a significant association between respondents’ knowledge regarding anti-malarial plants and gender (p-value =0.008) and occupation (p-value =0.025) but not with age (p-value =0.379), educational status (p-value =0.066), average monthly income (p-value =0.419), and religious affiliation (p-value =0.064) of respondents. A total of 37 plant species, belonging to 25 plant families were used to treat malaria. The most cited plant was Vernonia amygdalina Delile (64.8%), followed by Aleo vera (L.) Burm.f. (41.9%), Callistemon citrinus (Curt.) Stapf (29.3%), Mormodica feotida Schumach (22.0%), Cyphostemma adenocaule (A.Rich) wild & Drumm. (16.1%) and Eucalyptus globulus Labill. (15.4%). Among the plant parts, stem/bark (99.6%) and leaves (90.8%) were the most frequently used. The habits of the plants encountered were shrub, tree, herb, rhizomes and climber. The commonest modes of preparation included boiling in water, squeezing fresh leaves, crushing and pounding, and chewing. Oral route/drinking was the most common mode of administration, followed by steam inhaling of vapours from the aqueous extracts and skin bathing. Conclusion: The present study shows that the people living in Budondo sub-county traditionally use diverse flora to treat malaria. Further investigations are required to evaluate the potential toxicological effects and to isolate the active components of the reported plants which could be developed into effective, safe and affordable anti-malarial medicines in the future.
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    Mixed diets enhance edible grasshopper, ruspolia differens (Orthoptera: Tettigoniidae) performance during mass rearing
    (IntechOpen, 2021-10-18) Malinga, Geoffrey Maxwell; Opoke, Robert; Rutaro, Karlmax
    Mixing of diets is a notable dietary practice that is believed to improve performance-related characteristics such as growth, survival rate and egg-laying potential among insect herbivores. However, currently there is limited information regarding the performance of edible insects either on artificial and natural diets or their mixtures. This chapter reviewed recent literature on performance of a seasonally harvested and a widely consumed edible grasshopper, Ruspolia differens (Orthoptera: Tettigoniidae) reared on various artificial and natural diets. Our aim is to highlight diets and diet mixtures that results in the highest R. differens production. The results of the review show that R. differens performs better on mixed diets than on single or less diversified diets. In all reviewed studies, edible grasshoppers fed mixed diets either of natural plants or artificial diets achieved highest final weights, highest survival, highest fecundity and fastest development times than less diversified diets. The information is useful in designing technologies for large-scale rearing program for this species.
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    Prevalence of diarrhoea and associated risk factors among children under five years old in Pader District, northern Uganda
    (BMC Infectious Diseases, 2020-01-13) Omona, Stephen; Malinga, Geoffrey M.; Opoke, Robert; Openy, Geoffrey; Opiro, Robert
    Background: Diarrhoea remains a major cause of morbidity and mortality in children under 5 years in sub-Saharan Africa. Of the three East African countries, Uganda has the worst mortality rate in children < 5 years, with 22% of these deaths attributed to diarrhoea. For proper planning and implementation of control, an understanding of the prevalence and determinants of the disease is crucial. This study assessed the prevalence of diarrhoea and related risk factors among children < 5 years in Pajule Subcounty, Pader District in northern Uganda. Methods: A cross-sectional survey was conducted in April 2018, covering 244 randomly selected households having children < 5 years old in the study area. A semi-structured questionnaire was used to interview the households about diarrhoeal history in their children in the last 2 weeks preceding the survey, and on the risk factors predisposing children to diarrhoeal infections. Bivariate and multivariate logistic regression analyses with a 95% confidence interval and p < 0.05 was used to identify the risk factors associated with childhood diarrhoeal disease. Results: We found a prevalence of diarrhoea of 29.1% [95% CI (23.7–35.0)] among children < 5 years in Pajule Subcounty during the 2 weeks preceding the survey. Use of unprotected water sources, age of child caretaker, child weaning time and family size had significant associations with diarrhoeal morbidity. Conclusion: The prevalence of childhood diarrhoea among children < 5 years of age in rural settings of Pajule Subcounty was higher than the Ugandan national average. Use of unprotected water sources, age of child caretaker, child weaning time and family size were identified as predictors of diarrhoeal occurrence. These findings underscore the need for improving access to clean water and providing community health education as the best methods for fighting childhood diarrhoea in the study area.
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    Spatial distribution of tsetse flies and trypanosome infection status in a vector genetic transition zone in northern Uganda
    (Journal of Parasitology Research, 2022-06-01) Opiro, Robert; Okello, Allele Moses; Opoke, Robert; Oloya, Francis A.; Nakafu, Esther; Iwiru, Teresa; Echodu, Richard; Malinga, Geoffrey M.; Bargul, Joel L.; Opiyo, Elizabeth A.
    Background. Tsetse flies are vectors of the genus Trypanosoma that cause African trypanosomiasis, a serious parasitic disease of people and animals. Reliable data on the vector distribution and the trypanosome species they carry is pertinent for planning sustainable control strategies. This study was carried out to estimate the spatial distribution, apparent density, and trypanosome infection rates of tsetse flies in two districts that fall within a vector genetic transition zone in northern Uganda. Materials and Methods. Capturing of tsetse flies was done using biconical traps deployed in eight villages in Oyam and Otuke, two districts that fall within the vector genetic transition zone in northern Uganda. Trapped tsetse flies were sexed and morphologically identified to species level and subsequently analyzed for detection of trypanosome DNA. Trypanosome DNA was detected using a nested PCR protocol based on primers amplifying the internal transcribed spacer (ITS) region of ribosomal DNA. Results. A total of 717 flies (406 females; 311 males) were caught, all belonging to the Glossina fuscipes fuscipes species. The overall average flies/trap/day (FTD) was (). Out of the 477 (201 male; 276 females) flies analyzed, 7.13% (34/477) were positive for one or more trypanosome species. Three species of bovine trypanosomes were detected, namely, Trypanosoma vivax, 61.76% (21/34), T. congolense, 26.47% (9/34), and T. brucei brucei, 5.88% (2/34), and two cases of mixed infection of T. congolense and T. brucei brucei, 5.88% (2/34). The infection rate was not significantly associated with the sex of the fly (generalized linear model (GLM), , , ) and district of origin (, , , ). However, trypanosome infection was highly significantly associated with the fly’s age based on wing fray category (, , , ), being higher among the very old than the young. Conclusion. The relatively high tsetse density and trypanosome infection rate indicate that the transition zone is a high-risk area for perpetuating animal trypanosomiasis. Therefore, appropriate mitigation measures should be instituted targeting tsetse and other biting flies that may play a role as disease vectors, given the predominance of T. vivax in the tsetse samples.