Browsing by Author "Bazira, Joel"
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Item Assessing the quality of drinking water from selected water sources in Mbarara city, Southwestern Uganda(Plos one, 2024-03-28) Abaasa, Catherine N.; Ayesiga, Savino; Lejju, Julius B.; Andama, Morgan; Tamwesigire, Imelda K.; Bazira, Joel; Byarugaba, FrederickThis study assessed the physical, chemical, and microbiological quality with emphasis on risk score, source apportionment, geochemistry, feacal coliforms and water quality index of drinking water from selected water sources. A cross-sectional study was conducted in six villages in Mbarara city, south-western Uganda. Each selected source was inspected using a WHO-adopted sanitary inspection questionnaire. Each source’s risk score was calculated. Thirty-seven samples were taken from one borehole, nine open dug wells, four rain harvest tanks, and twenty-three taps. The values for apparent color and phosphate were higher than the permissible level as set by the World Health Organization and Ugandan standards (US EAS 12). The isolated organisms were Klebsiella spp. (8.11%), Citrobacter divergens (62.16%), Citrobacter fluendii (2.7%), E. coli (35.14%), Enterobacter aerogenes (8.11%), Enterobacter agglomerus (5.4%), Proteus spp. (2.7%), Enterobacter cloacae (13.5%), and Proteus mirabilis (2.7%). Twelve water sources (32.4%) had water that was unfit for human consumption that was unfit for human consumption (Grade E), Five sources (13.5%) had water that had a very poor index (Grade D), nine (24.3%) had water of poor index (Grade C), eight (21.6%) had water of good water index (Grade B), and only three (8.1%) had water of excellent water quality index (Grade A). The piper trilinear revealed that the dominant water type of the area were Mgso4 and Caso4 type. Gibbs plot represents precipitation dominance. PCA for source apportionment showed that well, tap and borehole water account for the highest variations in the quality of drinking water. These results suggest that drinking water from sources in Mbarara city is not suitable for direct human consumption without treatment. We recommend necessary improvements in water treatment, distribution, and maintenance of all the available water sources in Mbarara City, South Western Uganda.Item Bacteriological profile, antibiotic susceptibility and factors associated with neonatal Septicaemia at Kilembe mines hospital, Kasese District Western Uganda(Springer Nature, 2021-11-04) Zamarano, Henry; Musinguzi, Benson; Kabajulizi, Immaculate; Manirakiza, Godfrey; Guti, Walker; Muhwezi, Ivan; Ayan, Ahmed Hussein; Baweera, Agnes; Kabahinda, Boaz; Itabangi, Herbert; Bazira, Joel; Kabanda, TaseeraNeonatal septicaemia is one of the most common leading causes of neonatal morbidity and mortality in developing countries. It is estimated to affect more than 30 million people worldwide annually, potentially leading to 6 million deaths.The objective of the study was to determine the prevalence, bacteriological profile, antibiotic susceptibility and factors associated with neonatal septicaemia among neonates suspected to sepsis at Kilembe mines hospital. We conducted a descriptive cross-sectional study, where purposive sampling technique was used and blood was drawn from 122 neonates suspected to sepsis attending Kilembe Mines Hospital during the period (July to November 2020). Specimens were inoculated in Brain heart infusion broth, transported to Fortportal Regional Referral Hospital, plated daily up to 7 days on blood, chocolate, MacConkey agar and incubated in aerobic and 5% carbondioxide. Pure colonies were identified by Gram stain, biochemical tests and antibiotic sensitivities obtained by Kirby Bauer disc diffusion method. Associations were tested using Chi square with Fisher’s exact or Yates correction tests where necessary and statistical significance was set at P < 0.05. Stata (version 14) used for statistical analysis. Blood cultures were positive in 59.0% cases with 55.5% male and 44.4% female. EOS was present in 56.9% and LOS 43.1% of the cases. Gram negative (56.9%) organisms were most implicated with neonatal septicaemia than Gram positives ones (43.1%). Gram positive organisms exhibited better susceptibility to amikacin, linezolid and vancomycin but more resistant to ampicillin and gentamicin. Of the aminoglycosides, amikacin exhibited a verge over netilmicin and gentamicin against Gram negative isolates. Risk factors of neonatal septicaemia were mother’s age of ≥25 years, employed mothers, tertiary-level of education, SVD, ANC attendance of ≥4 times, UTI during pregnancy, PROMS, foul Smelling liquor, urban residence, neonatal birth weight of ≥2500 g, Apgar score 1st and 5th min ≥6 and resuscitation.Item The biological integrity of streams and channels draining into the Rwizi River system in Western Uganda(East African Journal of Science, Technology and Innovation, 2019) Atwebembeire, Jeninah; Andama, Morgan; Bazira, Joel; Bunny, Julius Lejju; Tumusiime, Julius; Wangalwa, Rapheal; Yatuha, JaneRwizi River, often called the life-line river, supports over five million people in Western Uganda and is a major contributor of freshwater to Lake Victoria. Surrounded by a large and rapidly growing population, the river has suffered anthropogenic disturbances whose impact on the integrity of the system is a subject of concern. Aquatic macroinvertebrates, used globally to monitor both short- and long-term environmental conditions, were thus used to assess the biological integrity of streams and channels draining into the river system. Macroinvertebrates were sampled for six months in 2017 encompassing the wet and dry seasons using the kick net sampling method. The macroinvertebrates were identified morphologically using peer reviewed identification keys and their pollution sensitivity scored using the Tanzanian River Scoring System (TARISS). The Shannon diversity index was computed per site and related to average score per taxon (ASPT). We collected a total of 5442 macroinvertebrates belonging to 54 families dominated by Chironomidae (29.1%). Macroinvertebrate diversity increased with ASPT (r = 0.57; N = 131; P = 0.000). The water quality was generally poor and was not affected by the season (t = 1.03; df = 64; P = 0.303) though sites had different water quality (F = 11.32; df = 20; P = 0.000) attributed the degree of anthropogenic disturbance. We concluded that river Rwizi system is highly degraded and thus recommend restoration of the entire catchment. Aquatic macroinvertebrates are good indicators of long-term conditions but less sensitive to short-term changes. Multiple approaches, biological and chemical, are encouraged during the restoration process.Item Investigating metabolic and molecular ecological evolution of opportunistic pulmonary fungal coinfections: protocol for a laboratory-based cross-sectional study(JMIR Publications, 2023-04-09) Njovu, Israel Kiiza; Nalumaga, Pauline Petra; Ampaire, Lucas; Nuwagira, Edwin; Mwesigye, James; Musinguzi, Benson; Kassaza, Kennedy; Taseera, Kabanda; Mukasa, James Kiguli; Bazira, Joel; Iramiot, Jacob Stanley; Baguma, Andrew; Bongomin, Felix; Kwizera, Richard; Achan, Beatrice; Cox, Michael J; King, Jason S; May, Robin; Ballou, Elizabeth R; Itabangi, HerbertBackground: Fungal-bacterial cocolonization and coinfections pose an emerging challenge among patients suspected of having pulmonary tuberculosis (PTB); however, the underlying pathogenic mechanisms and microbiome interactions are poorly understood. Understanding how environmental microbes, such as fungi and bacteria, coevolve and develop traits to evade host immune responses and resist treatment is critical to controlling opportunistic pulmonary fungal coinfections. In this project, we propose to study the coexistence of fungal and bacterial microbial communities during chronic pulmonary diseases, with a keen interest in underpinning fungal etiological evolution and the predominating interactions that may exist between fungi and bacteria. Objective: This is a protocol for a study aimed at investigating the metabolic and molecular ecological evolution of opportunistic pulmonary fungal coinfections through determining and characterizing the burden, etiological profiles, microbial communities, and interactions established between fungi and bacteria as implicated among patients with presumptive PTB. Methods: This will be a laboratory-based cross-sectional study, with a sample size of 406 participants. From each participant, 2 sputa samples (one on-spot and one early morning) will be collected. These samples will then be analyzed for both fungal and bacterial etiology using conventional metabolic and molecular (intergenic transcribed spacer and 16S ribosomal DNA–based polymerase chain reaction) approaches. We will also attempt to design a genome-scale metabolic model for pulmonary microbial communities to analyze the composition of the entire microbiome (ie, fungi and bacteria) and investigate host-microbial interactions under different patient conditions. This analysis will be based on the interplays of genes (identified by metagenomics) and inferred from amplicon data and metabolites (identified by metabolomics) by analyzing the full data set and using specific computational tools. We will also collect baseline data, including demographic and clinical history, using a patient-reported questionnaire. Altogether, this approach will contribute to a diagnostic-based observational study. The primary outcome will be the overall fungal and bacterial diagnostic profile of the study participants. Other diagnostic factors associated with the etiological profile, such as incidence and prevalence, will also be analyzed using univariate and multivariate schemes. Odds ratios with 95% CIs will be presented with a statistical significance set at P<.05. Results: The study has been approved by the Mbarara University Research Ethic Committee (MUREC1/7-07/09/20) and the Uganda National Council of Science and Technology (HS1233ES). Following careful scrutiny, the protocol was designed to enable patient enrollment, which began in March 2022 at Mbarara University Teaching Hospital. Data collection is ongoing and is expected to be completed by August 2023, and manuscripts will be submitted for publication thereafter. Conclusions: Through this protocol, we will explore the metabolic and molecular ecological evolution of opportunistic pulmonary fungal coinfections among patients with presumptive PTB. Establishing key fungal-bacterial cross-kingdom synergistic relationships is crucial for instituting fungal bacterial coinfecting etiology.Item The physico-chemical quality of effluents of selected sewage treatment plants draining into river Rwizi, Mbarara Municipality, Uganda(Journal of Water Resource and Protection, 2019) Atwebembeire, Jeninah; Andama, Morgan; Yatuha, Jane; Lejju, Julius Bunny; Kagoro-Rugunda, Rapheal; Bazira, JoelAs population increases in urban areas, the domestic and industrial activities increase resulting in an increase in the volumes of wastewater and anthropogenic pollution, hence posing a threat to public health and environment. This study assessed the physical chemical quality of two main sewage plant effluents discharging into River Rwizi. Effluent water samples were analyzed for dissolved oxygen, colour, turbidity, total suspended solid, total iron, phosphates, alkalinity, magnesium, calcium carbonate, temperature, pH, ammonium, electrical conductivity, chloride and nitrates. Parameters were analyzed following standard methods of APHA (1985). The values obtained were compared with EPA (2001), NWSC (2015) and NEMA (1999) standards for waste water. Results showed that the mean values most of the parameters tested were higher than the recommended EPA, NWSC and NEMA standards. The mean colour was 1627.67°C and 1414.33°C in Kakoba and Taso sewage effluents respectively compared to EPA (20-150), NWSC (500) and NEMA (300) standards (p > 0.05). The mean alkalinity was 1390.17 mg/l and 1308.33 mg/l for Kakoba and Taso respectively compared with EPA (400) and NWSC (800) standards. DO had a mean concentration of 68.27 mg/l and 63.03 mg/l in Taso and Kakoba respectively compared to EPA and NEMA standard of 5 mg/l. Mg was 243.29 mg/l and 246.49 mg/l in Kakoba and Taso sewage effluents respectively compared to NEMA standard for waste water of 100 mg/l (p > 0.05). The mean pH was 8.26 and 8.16 in Taso and Kakoba sewage effluents respectively compared to NWSC and NEMA standard of 6.0 - 8.0. Phosphate mean concentration levels were 32.2 mg/l and 27.11 mg/l for Taso and Kakoba respectively compared to standards of EPA (0.5 - 0.7 mg/l) and NEMA (10 mg/l). NO3 was 10 mg/l and 5.83 mg/l in Kakoba and Taso sewage effluents respectively compared to NWSC maximum permissible limit of 5 mg/l. The mean NH4 concentration was 385.33 mg/l (Kakoba) and 50.0 mg/l (Taso) compared to the EPA guideline range (0.2 - 4 mg/l). Chloride (Cl) had a mean of 833.33 mg/l in Kakoba compared to EPA (250 mg/l), NWSC and NEMA (500 mg/l) standards. Therefore the study recommends for effective treatment of waste effluents from Kakoba and Taso sewage treatment plants before recycling in order to avoid pollution of river Rwizi.Item The physico-chemical quality of streams and channels draining into river Rwizi, South Western Uganda(Journal of Water Resource and Protection, 2018) Atwebembeire, Jeninah; Bazira, Joel; Kagoro, Grace; Yatuha, Jane; Andama, Morgan; Lejju, Julius BunnyThere is an increased human population along the catchment area of river Rwizi who are engaged in various activities with potential to contaminate river Rwizi especially along the streams. Studies have been done to examine the physico-chemical quality of the mainstream river Rwizi in Mbarara Municipality. However, the comprehensive source of contamination may be beyond the municipality and yet the streams have not been investigated to substantiate their contribution on quality degradation of the river and this formed the basis of the study. This study determined the physico-chemical parameters of streams draining into river Rwizi. Raw water was purposively selected from streams in the upstream, midstream and downstream sections of the river. Water samples were analyzed for 15 physico-chemical parameters including dissolved oxygen (DO), colour, turbidity, total suspended solid (TSS), total iron (Fe), phosphates (PO3-4), alkalinity, magnesium (Mg), calcium carbonate (CaCO3), temperature, pH, ammonium (NH+4), electrical conductivity (EC), chloride (Cl) and nitrates (NO3). The water samples were analyzed following the standard methods of American Public Health Association and standard operating manual (1985). The obtained values were also compared with the international Environmental Protection Agency (EPA, 2001) guidelines for water quality and the National Environment Management Authority (NEMA, 1999) standards for waste water. The results showed that most of the streams in the downstream generally recorded the highest levels (p < 0.05) of the physico-chemical parameters followed by the midstream and lastly the upstream. Streams in the downstream recorded the highest levels in most of the parameters tested i.e. bus park stream (temperature, 27.6°C; colour, 431.17 TCU; TSS, 99.33 mg/l; alkalinity, 468.33 mg/l; Mg, 121.89 mg/l; CaCO3, 588.67 mg/l; Cl, 333.33 mg/l), Kikutu stream (turbidity, 123.58 NTU; EC, 698 μs/cm; DO, 55.73 mg/l; PO3-4, 12.85 mg/l), Kakyeka upper (pH, 10.52; NH+4, 46.67 mg/l), Rwentondo (Fe, 3.44 mg/l) and Kakyeka GBK (NO3, 10.83 mg/l). Most of the parameters downstream were higher than the EPA guideline (temperature, 25°C; colour, 20 - 150 TCUs; TSS, 50 mg/l; alkalinity, 400 mg/l; Cl, 250 mg/l; DO, 5 mg/l; PO3-4, 0.5 - 0.7 mg/l; pH, 5.5 - 9.0; NH+4, 0.2 - 4 mg/l; Fe, 0.2 - 2.0 mg/l) and NEMA standards (colour, 300 TCUs; Mg, 100mg/l; DO, 5mg/l; PO3-4, 10 mg/l; pH, 6.0 - 8.0). On the other hand, the streams in the upstream registered the lowest values of the parameters i.e. Kibimba (temperature, 17.28°C; EC, 31 μs/cm; PO3-4, 0.24 mg/l), Kasharara (colour, 15.17 TCU; TSS, 2.5 mg/l; pH, 6.23; alkalinity, 19.67 mg/l; DO, 9.99 mg/l; Mg, 4.41 mg/l; CaCO3, 23.17 mg/l) and Karungu (turbidity, 7.02 mg/l; Fe, 0.12 mg/l). Calcium carbonate hardness of the waters ranged from soft to excessively hard across the stream. The variations of the parameters detected in the streams in the downstream could reach adverse conditions if no intensive measures are taken to regulate the different anthropogenic activities within the catchment of river Rwizi.Item Status of pulmonary fungal pathogens among individuals with clinical features of pulmonary tuberculosis at Mbarara University Teaching Hospital in Southwestern Uganda(SAGE Publications, 2021-08-31) Njovu, Israel Kiiza; Musinguzi, Benson; Mwesigye, James; Kassaza, Kennedy; Turigurwa, Joseph; Nuwagira, Edwin; Bazira, Joel; Kabanda, Taseera; Mpeirwe, Moses; Ampaire, Lucas; Mutekanga, Andrew; Kiguli, James; Achan, Beatrice; Itabangi, HerbertPulmonary mycoses are important diseases of the respiratory tract caused by pulmonary fungal pathogens. These pathogens are responsible for significant morbidity and mortality rates worldwide; however, less attention has been paid to them. In this study we determined the prevalence of pulmonary fungal pathogens among individuals with clinical features of pulmonary tuberculosis at Mbarara Regional Referral Hospital. This was a hospital based cross sectional survey. Sputum samples were collected from each study participant. For each sample, the following tests were performed: Sabouraud dextrose agar for fungal culture, GeneXpert for Mycobacteria tuberculosis (MTB) and potassium hydroxide for fungal screening. Filamentous fungal growth and yeasts were further examined with lactophenol cotton blue staining and germ tube respectively. Out of 113 study participants, 80 (70.7%) had pulmonary fungal pathogens whilst those with pulmonary tuberculosis numbered five (4.4%). Candida albicans [21 (22.58%)] and Aspergillus species [16 (17.20%)] were the pathogens most identified among others. Two (1.7%) TB GeneXpert positive participants had fungal pathogens isolated from their sputum samples. We established a prevalence of 57 (71.3%) for pulmonary fungal pathogen (PFP) isolates, three (60.0%) for MTB in HIV positive patients and 18 (22.5%) for PFP, and zero (0.0%) for MTB in HIV negative patients. On the other hand, two (100%) HIV positive patients had both PFP isolates and MTB.Our findings highlight the diversity of neglected pulmonary fungal pathogens whose known medical importance in causing pulmonary mycoses cannot be overemphasised. Therefore this presents a need for routine diagnosis for pulmonary mycoses among TB suspects and set-up of antimicrobial profile for pulmonary fungal isolates to support clinical management of these cases.