Browsing by Author "Amutuhaire, Treasure Angie"

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    Pharmacognostical evaluation and description of the leaf and root bark of Citropsis articulata Swingle & Kellerman
    (Elsevier, 2024-09-14) Amaza, Ronald Anyase; Amutuhaire, Treasure Angie; Tusiimire, Jonans; Amito, Vivian Sharon; Ajayi, Clement Olusoji; Angupale, Jimmy Ronald; Abdelgadir, Abdelgadir Alamin; Anywar, Godwin; Ogwang, Patrick Engeu
    Background: Citropsis articulata Swingle & Kellerman (Family Rutaceae) is a renowned African traditional medicinal plant for the management of erectile dysfunction. The root bark is more commonly used than the leaves. Little has been done to evaluate the pharmacognostical parameters of both parts. Purpose: The aim of this study was to determine the pharmacognostical parameters of C. articulata leaves and root bark. Methods: The plant parts were collected, washed, dried, and extracted by decoction and soxhlation. The World Health Organisation quality control methods for herbal materials were generally followed for the pharmacognostical evaluation of the root bark and leaf. This involved macroscopic and microscopic analysis, physicochemical analysis, hemolytic activity, and phytochemical analysis. Results: Macroscopically, the distillate of the root bark showed a fluorescent blue colour under ultra violet light of wavelength 254 nm. Microscopic examination revealed the presence of cellulose, lignified, suberized and cuticular cell walls. Numerous aleurone grains were noted in the root bark. Phytochemical analysis showed the presence of reducing sugars, free amino acids, L-arginine, coumarin, glycosides, phenols, phytosterols, saponins, steroids, tannins, terpenoids, alkaloids and flavonoids. Thin layer and high performance liquid fingerprints were also developed. The C. articulata root bark decoction and Soxhlet extracts contained 12.35 ± 0.05, and 110.00 ± 0.03 while the leaf decoction and Soxhlet extracts contained 4.97 ± 0.03 and 39.65 ± 0.00 mg/g diosgenin equivalent saponins respectively. The L-arginine content for the dried root bark decoction and Soxhlet was 2681 ± 7.29, 1660 ± 2.99 µg/g, while that for the leaf decoction and Soxhlet extracts was 135.60 ± 8.28, and 130.20 ± 2.83 µg/g respectively. Conclusion: This study distinguished the root bark and leaf of Citropsis articulata and offered vital information for the quality control and standardization of the plant materials.