Liu, YangheYin, SijieChen, ShiyueZhang, ChunleiLubanga, NasifuTai, PingXiong, MengqiuFan, BoyueYang, XinchengXia, XinyiHou, PanfeiHe, Bangshun2025-11-252025-11-252025-10-15Liu, Y., Yin, S., Chen, S., Zhang, C., Lubanga, N., Tai, P., Xiong, M., Fan, B., Yang, X., & Xia, X. (2025). Rapid detection of Helicobacter pylori and its virulence genes by fluorescence-chromogenic double-indicator LAMP (FC-LAMP). Clinica Chimica Acta, 120667.0009-8981https://dir.muni.ac.ug/handle/20.500.12260/808Helicobacter pylori (H. pylori) is a risk factor of gastrointestinal diseases. Vacuolating cytotoxin A (VacA) is a key component of the pathogenicity of H. pylori for gastric diseases, and detection for virulence genotypes facilitates precise clinical diagnosis and individual treatment. Hence, a more accurate, convenient, and highly sensitive detection method is urgently needed. In this study, we developed a method based on fluorescence-colorimetric dual-indicator loop-mediated isothermal amplification (FC-LAMP), allowing for naked-eye readout, using a constant temperature water bath within 40 min in clinical settings. Such an excellent sensitivity and specificity, and user-friendly operation approach achieved a limit of 10−6 ng/μl for 16 s rRNA detection of H. pylori, and 100 % specificity for 55 clinical gastric fluid samples compared with the immunofluorescence staining (IFS) and quantitative polymerase chain reaction (qPCR) as reference methods. In short, this study offers a new strategy for detection of H. pylori and its virulence genotype in primary hospitals and in limited resources settings, facilitating individualized precision eradication of H. pylori.enHelicobacter pyloriLoop-mediated isothermal amplificationFluorescence-colorimetric dual-indicatorVirulence genesArticle