Laker, FortunateAgaba, ArnoldAkatukunda, AndrewGazet, RobertBarasa, JoshuaNanyonga, SarahWendiro, DeborahWacoo, Alex Paul2018-08-142018-08-142018-05-16Laker, F., Agaba, A., Akatukunda, A., Gazet, R., Barasa, J., Nanyonga, S., ... & Wacoo, A. P. (2018). Utilization of Solid Waste as a Substrate for Production of Oil from Oleaginous Microorganisms. Journal of lipids, 2018.https://dir.muni.ac.ug/handle/20.500.12260/238The overwhelming demand of oil and fats to meet the ever increasing needs for biofuel, cosmetics production, and other industrial purposes has enhanced a number of innovations in this industry. One such innovation is the use of microorganisms as alternative sources of oil and fats. Organic solid waste that is causing a big challenge of disposal worldwide is biodegradable and can be utilized as substrate for alternative oil production.The study evaluated the potential of isolated yeast-like colonies to grow and accumulate oil by using organic solid waste as substrate. Of the 25 yeast-like colonies isolated fromthe soil samples collected fromthree different suburbs in Kampala district, Uganda, 20 were screened positive for accumulation of lipid but only 2 were oleaginous. The NHC isolate with the best oil accumulation potential of 48.8% was used in the central composite design (CCD) experiments. The CCD experimental results revealed a maximum oil yield of 61.5% from 1.25 g/L cell biomass at 10 g/L of solid waste and temperature of 25∘C. The study revealed that organic solid waste could be used as a substrate for microbial oil production.Solid WasteOleaginous MicroorganismsSubstrateArticle